ProGP399 (TfsA (S-layer protein))

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ProGP ID ProGP399 (TfsA (S-layer protein))
Validation Status Characterized
Organism Information
Organism NameTannerella forsythia ATCC 43037
Domain Bacteria
Classification Phylum : Bacteroidetes
Class : Bacteroidia
Orders : Bacteroidales
Family : Tannerellaceae
Genus : Tannerella
Species : forsythia
Strain : ATCC 43037
Taxonomic ID (NCBI) 203275
Genome Information
GenBank GQ856797
EMBL GQ856797
Organism Additional Information Tannerella forsythia is an oral pathogen causing chronic periodontitis. It forms polymicrobial subgingival plaque biofilm along with Porphyromonas gingivalis and Treponema denticola.
Gene Information
Gene NametfsA (TF2661-2)
Protein Information
Protein NameTfsA (S-layer protein)
UniProtKB/SwissProt ID Q1JQR6
EMBL-CDSAAR82922.1
UniProtKB Sequence >tr|Q1JQR6|Q1JQR6_TANFO Surface layer protein A OS=Tannerella forsythia GN=tfsA PE=4 SV=1 MNKKVFTLLAASLMLFMTAFMADARPFYGDAVKYLPEGMGKGAYHLKVTLQTATGPVDKF LAMDEFGYITLVDSAFIYGASVPADSSYRRLRAAMWCVNVTKPELYGKVPGFAFTNKEYG AGLEFEYESSMFKKMCAGMDVHSQRDLPLVGGNLGIWKFSRTYNTVPLEQEQYLAIEVKP DYYMTFAINKTCGASDSVRLVVAHKDEFDVNADFYKKDLLKFTLVNARPRVLTAKDFNTM MYEYSKEEYRQLEFSQKVSAGQVNPFEQPLMAEDVTGTTGDKQYLNLKNKDGKYVTALAG NTEENYVNDLGYRYPKIVTGGKSTSQHSDWRLVYYPSEDSLVINVRRLNHMVEGDNEDNM TYLDGEYDGLFNQTIWNFLTVRMQDLSAGERVVTVRNKPANVRGFFNINSCVLYDKNRTT VEGDLYTIKDKDGRYLVVPMYAGNFTPQWLRLDENEVANMTPSYQWYVEQVNEGSSISKI HLINREFDDIRIEYVQIYNDYELFKGVWWRLDPKNKKKDYSPVMGRNFVNKESFIRVPRP YRNDPYLGYRFFSRKPSQFGKEDGGIAEATDSLNWFAYTFQYLSGLSGNHYMGFDEKASN KDTILYVLKGEKTYFQLVIPDSLRKEAYGTERYGIGWTSNSPLKSKFTKKGTDDYIAPLE RWYYHLQINDYWKFKRNEYYLVLDDNGRYGYTPEVIANARKLNKAKFYLRFTYQVDGKNY YALLDRIDLSNFHYYTDITGLPITDTVRVHDTSHGSIKNNSFGVVHASVDDLNNYVRSQP KTVGGQRVSTFAISCETEPLYRRFNTTDESAIGSDNPDTLKFHRTAVGKEREYLYEDAHS IYSYDIKRLPGKNHSSVNFLGVQNEDDAIANKDKDQVFKRHLDTDWAIYVDTAYVNRGTG HIKPQYLLAVDPKFGYLGCPVCGEDQLNRPFVYARYLRNQTDSARLAGHPDGTIVDKDYI WDAKWERLSFVPAIHAGDTLYILNGRPIEAFIQKKKEADGTETEYVNYKKLRNDSKVKIV FLGNNKHKDEVFSFRFTEVGGGSNKDFMIESETTRRDVTKGRMIAPMNGGWVKIQNGVPV ISRGAYTDWIAEAETWNVKMTKKSPLANAEASVTDVKVVAGAGEVMIRNASGKKVTVSNI LGQTIAKVVLTSDNQTIAAPQGVVVVAIEGENAVKAVVK
Sequence length 1179AA
Function S-layer helps T. forsythia in adhesion/invasion to human gingival epithelial cells and to epidermal carcinoma cells of the mouth. The host immune response to this pathogen is attenuated by the S-layer after immune evasion.
Glycosylation Status
Glycosylation Type O- (Ser/Thr) linked
Experimentally Validated Glycosite(s) in Full Length ProteinS197, T819, S943, T979
Glycosite(s) Annotated Protein Sequence >tr|Q1JQR6|Q1JQR6_TANFO Surface layer protein A OS=Tannerella forsythia GN=tfsA PE=4 SV=1 MNKKVFTLLAASLMLFMTAFMADARPFYGDAVKYLPEGMGKGAYHLKVTLQTATGPVDKF LAMDEFGYITLVDSAFIYGASVPADSSYRRLRAAMWCVNVTKPELYGKVPGFAFTNKEYG AGLEFEYESSMFKKMCAGMDVHSQRDLPLVGGNLGIWKFSRTYNTVPLEQEQYLAIEVKP DYYMTFAINKTCGASDS*(197)VRLVVAHKDEFDVNADFYKKDLLKFTLVNARPRVLTAKDFNTM MYEYSKEEYRQLEFSQKVSAGQVNPFEQPLMAEDVTGTTGDKQYLNLKNKDGKYVTALAG NTEENYVNDLGYRYPKIVTGGKSTSQHSDWRLVYYPSEDSLVINVRRLNHMVEGDNEDNM TYLDGEYDGLFNQTIWNFLTVRMQDLSAGERVVTVRNKPANVRGFFNINSCVLYDKNRTT VEGDLYTIKDKDGRYLVVPMYAGNFTPQWLRLDENEVANMTPSYQWYVEQVNEGSSISKI HLINREFDDIRIEYVQIYNDYELFKGVWWRLDPKNKKKDYSPVMGRNFVNKESFIRVPRP YRNDPYLGYRFFSRKPSQFGKEDGGIAEATDSLNWFAYTFQYLSGLSGNHYMGFDEKASN KDTILYVLKGEKTYFQLVIPDSLRKEAYGTERYGIGWTSNSPLKSKFTKKGTDDYIAPLE RWYYHLQINDYWKFKRNEYYLVLDDNGRYGYTPEVIANARKLNKAKFYLRFTYQVDGKNY YALLDRIDLSNFHYYTDITGLPITDTVRVHDTSHGSIKNNSFGVVHASVDDLNNYVRSQP KTVGGQRVSTFAISCETEPLYRRFNTTDESAIGSDNPDT*(819)LKFHRTAVGKEREYLYEDAHS IYSYDIKRLPGKNHSSVNFLGVQNEDDAIANKDKDQVFKRHLDTDWAIYVDTAYVNRGTG HIKPQYLLAVDPKFGYLGCPVCGEDQLNRPFVYARYLRNQTDS*(943)ARLAGHPDGTIVDKDYI WDAKWERLSFVPAIHAGDT*(979)LYILNGRPIEAFIQKKKEADGTETEYVNYKKLRNDSKVKIV FLGNNKHKDEVFSFRFTEVGGGSNKDFMIESETTRRDVTKGRMIAPMNGGWVKIQNGVPV ISRGAYTDWIAEAETWNVKMTKKSPLANAEASVTDVKVVAGAGEVMIRNASGKKVTVSNI LGQTIAKVVLTSDNQTIAAPQGVVVVAIEGENAVKAVVK
Sequence Around Glycosites (21 AA) AINKTCGASDSVRLVVAHKDE
ESAIGSDNPDTLKFHRTAVGK
YARYLRNQTDSARLAGHPDGT
SFVPAIHAGDTLYILNGRPIE
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Technique(s) used for Glycosylation DetectionPro-Q Emerald carbohydrate staining
Protein Glycosylation- Implication Here, the protein O-glycosylation system is needed for creating a rich glycoproteome that is possibly related to the virulence of this bacterium.
Glycan Information
Glycan Annotation An oligosaccharide with the structure 4-Me-β-ManpNAcCONH2-(1→3)-[Pse5Am7Gc-(2→4)-]-β-ManpNAcA-(1→4)-[4-Me-α-Galp-(1→2)-]-α-Fucp-(1→4)-[-α-Xylp-(1→3)-]-β-GlcpA-(1→3)-[-β-Digp-(1→2)-]-α-Galp.
GlyTouCan G58135UN
Protein Glycosylation linked (PGL) gene(s)
Additional CommentUntil now, T. Forsythia is the only known Gram-negative bacterium that is covered with a glycosylated S-layer, with, again uniquely, two S-layer glycoproteins being simultaneously present.
Literature
Year of Identification2011
Year of Identification Month Wise2011.2.18
Year of Validation 2011
ReferencePosch, G., Pabst, M., Brecker, L., Altmann, F., Messner, P. and Schäffer, C., 2011. Characterization and scope of S-layer protein O-glycosylation in Tannerella forsythia. Journal of Biological Chemistry, 286(44), pp.38714-38724.
Corresponding Author Christina Schaffer
ContactDepartment of NanoBiotechnology, NanoGlycobiology, Vienna Institute of BioTechnology, Universität für Bodenkultur Wien, Muthgasse 11, A-1190 Vienna, Austria.